Ema adult cam


15-Sep-2017 05:53

Various foods have been pasteurized to kill bacteria while minimizing the denaturation of food components such as proteins.

Therefore, the bacteria present in clinical samples and foods may be injured.

The PCR amplification of DNA from dead bacteria was inhibited by cross-linking action (23, 27-29), and the PCR signal from dead bacteria was reduced to 1/300 to 1/1,000 (23, 27, 29).

It has been reported that pasteurized milk contains 10 cells/ml of injured/dead bacteria (1, 30).

We employed EMA, which cleaves DNA after photoactivation (32), DNA gyrase/topoisomerase IV poison (8, 15, 24, 37), and mammalian topoisomerase (I and II) poisons (3, 7, 14, 18, 19, 34). Thus, we used DNA gyrase/topoisomerase IV and mammalian topoisomerase poisons (here abbreviated as T-poisons) together with EMA Light. Herein, we report that EMA Light randomly cleaved chromosomal DNA of heat-treated, but not live, , DNA amplification was not completely suppressed by EMA Light only.The European Union Clinical Trials Register allows you to search for protocol and results information on: Learn more about the EU Clinical Trials Register including the source of the information and the legal basis.

Subscribe to this Search To subscribe to the RSS feed for this search click here .Hyperthermophilic enzymes are reported to be maintained in the bacteria of pasteurized milk (9, 11, 36).



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